Research Interests

Our research is focused on the development of integrative structural methods, combining experimental data with bioinformatics and modelling techniques, to characterise macromolecular machines. Structural models are further analysed to understand their function and design new experiments. The group is particularly interested in using data from cryoEM, cryoET, and Mass Spectrometry. In collaboration with experimentalists, specific systems we currently study include HSV-1 and HCMV sub-complexes, ligand-gated ion channels, and microtubule complexes. 

Selected recent publications

1. •Hernández Durán A, et al. Consensus clustering of herpesvirus protein interaction networks provides insights into their evolutionary relationship with bacteriophages. PLoS Biol, 2019 (in press).
   

2. •Bullock JMA, Sen N, et al. Modelling protein complexes using restraints from cross-linking mass spectrometry. Structure, 26:1015, 2018.
   

3. •Locke J, Joseph AP, et al. “Molecular basis of human kinesin-8 function and inhibition.” Proc Natl Acad Sci USA 114:E9539, 2017.
   

4. •Joseph AP, et al. “Integrative modelling of cellular assemblies.” Curr Opin Struct Biol 46:102, 2017.
   

5. •Bullock JMA, Schwab J, et al. “The importance of non-accessible crosslinks and solvent accessible surface distance in modelling proteins with restraints from crosslinking mass spectrometry.” Mol Cell Proteomics, 2016, 15:10.1074.
   

6. •Joseph AP, Malhotra S, et al. “Refinement of atomic models in high resolution EM reconstructions using Flex-EM and local assessment.” Methods 100:42, 2016.